Diagnosis of Paratuberculosis by Fecal Culture Followed by Nested-Polymerase Chain Reaction
نویسندگان
چکیده
منابع مشابه
SPECIFIC AMPLIFICATION OF ASPERGILLUS FUMlGATUS DNA BY POLYMERASE CHAIN REACTION
Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus s...
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In view of the importance of early diagnosis of tuberculous meningitis (TBM), the efficiency of the polymerase chain reaction (PCR), one of the most reliable and sensitive DNA-based assays, was compared with conventional methods (acid-fast microscopy and culture) for the detection of M. tuberculosis in cerebrospinal fluid (CSF) specimens from patients suspected of TBM. Of the 29 CSF specim...
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The early detection of scrub typhus in Thailand by nested polymerase chain reaction (PCR) is presented. The diagnosis of scrub typhus, from clinical samples obtained from hospitals in the northern part of Thailand, by nested PCR was compared to immunofluorescence (IF) and Weil-Felix (WF) tests. The primer pairs used for the nested PCR were designed on the basis of the nucleotide sequence of the...
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Canine coronavirus (CCV) is a single-stranded RNA virus belonging to the Coronaviridae family and is responsible for generally mild gastroenteritis in pups. Infected dogs shed CCV in feces for 6-9 days, but shedding can be prolonged in some pups. The virus content of feces is very high at the time of clinical signs. Electron microscopic (EM) examinations of fecal suspensions or isolation in tis...
متن کاملPolymerase Chain Reaction for the Diagnosis of Tuberculosis
Dear Editor-in-Chief We read with interest the study by Khazaei et al. (1) in which the authors have nicely concluded that PCR is more sensitive test than Ziehl-Neelsen staining and histo-pathological examination for the diagnosis of tuberculosis (TB). They have rightly pointed to use PCR, selectively, in acidfast bacilli negative paucibacillary forms of TB. However, we intend to highlight few...
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ژورنال
عنوان ژورنال: International Journal of Current Microbiology and Applied Sciences
سال: 2020
ISSN: 2319-7692,2319-7706
DOI: 10.20546/ijcmas.2020.905.194